A consolidated bioprocess integrating enzyme production, saccharification and fermentation was applied for bioethanol production. Mixed solid-state fermentation of alkali-pretreated sugarcane bagasse (SCB) supplemented with wheat bran (WB) to produce a compound enzyme for saccharification by Trichoderma and Penicillium was carried out, and at 72 h, the obtained sugars concentration was 20.190 g/L. Then the mixture was further converted to ethanol by inoculating Saccharomyces cerevisiae. After 24 h, the maximum ethanol concentration 5.825 g/L (40.84% of theoretical yield) was achieved. At 144 h, the produced 8-glucosidase activity and fungal biomass were 0.483 IU/mL and 0.204 g/g, respectively. The consolidated bioprocess avoided enzyme preparation, saved much water/energy consumption and reduced equipment investment, which indicated that it might be an effective approach for the economic production of bioethanol. (C) 2015 Elsevier Ltd. All rights reserved.