Fe60Co40 nanoparticles with an average particle size of 30 nm were successfully synthesized in gram scale batches using the modified polyol process. The X-ray diffraction and microstructure studies clearly show the formation of the alloy nanoparticles. The saturation magnetization for the gram scale synthesized Fe60Co40 alloy nanoparticles is in the range of 190-205 emu/g at room temperature. The as-synthesized nanoparticles were used to fabricate transmission lines on FR4 substrate to perform radio frequency (RF) characterization of the nanoparticles at ISM RF bands of interest (all in GHz range). The complex permeability extraction of composite Fe60Co40 nanoparticles were performed using perturbation technique applied to microstrip transmission lines by relative measurement of full two port scattering parameter with respect to a baseline FR4 substrate. The extracted results show attractive characteristics for small size antennas and filters. (C) 2014 Elsevier B.V. All rights reserved.