In this report, we have studied the adsorption behavior of Fe3O4 nanoparticles into dipalmitoylphos-phatidylcholine (DPPC) monolayer. Adsorption kinetics (pi-t) process as well as the surface pressure (pi-A) isotherms were monitored by Langmuir Wilhelmy plate. The measurement data indicated the Fe3O4 nanoparticles incorporated into the monolayer at the air-water interface. The lifting area/molecule isotherms of DPPC monolayers increased with the increasing concentration of Fe3O4 in the subphase, however, the values of elasticity reduced. The curves of pi-t were well fitted by single exponential association equation. Observation by atomic force microscopy (AFM) on monolayers extracted at 5 mN/m and 15 mN/m suggested that the different interaction of Fe3O4 with DPPC monolayer depended on surface pressure of monolayers and concentration in the subphase. The results of observations were in agreement with the fitted results. (C) 2015 Elsevier B.V. All rights reserved.