The production and properties of the indium-tin-oxide (ITO) electrodes functionalized by Au nanopartide (NP) arrays of a relatively large area formed by pulsed laser nanostructuring of thin gold films are reported and discussed. The SEM inspection of modified electrodes reveals the presence of the nearly spherical and disc-shaped particles of dimensions in the range of 40-120 nm. The NP-array geometry can be controlled by selection of the laser processing conditions. It is shown that particle size and packing density of the array are important factors which determine the electrode performance. In the case of NP-modified electrodes the peak current corresponding to the glucose direct oxidation process shows rise with increasing glucose concentration markedly higher comparing to the reference Au disc electrode. The detection limit reaches 12 mu M and linear response of the sensor is observed from 0.1 to 47 mM that covers the normal physiological range of the blood sugar detection. (C) 2015 Elsevier B.V. All rights reserved.