Improper management of cattle manure and poultry litter from confined animal farming are usually source of water pollution. However, appropriate application of these products on switchgrass (Panicum virgatum L.) field can enhance biomass yield and promote nutrient recycling. We evaluated the effects of harvest frequency and nutrient sources on yield and quality of switchgrass for biofuel feedstock. The experiment was carried out at Perkins and Lake Carl Blackwell, Oklahoma, from 2009 to 2011 using split plot design with four replications. The main plot treatments were two harvest frequencies single (June), and twice (June and November). The subplot treatments were nutrient sources: 1) cattle manure (CM), 2) poultry litter (PL), 3) urea (nitrogen at 150 kg ha(-1)), 4) combined chemical fertilizer (nitrogen-P2O5-K2O) with nitrogen at 150 kg ha(-1), P2O5 at 40 kg ha(-1), and K2O at 20 kg ha(-1), 5) inter-seeded Crimson clover (Trifolium incarnatum L.), and 6) control. Mean biomass yield was higher (12.4 Mg ha(-1)) in 2010 than other years possibly due to optimum moisture and temperature in 2010. At Perkins in 2010, application of CM and PL increased biomass yield significantly by 30 and 23% compared with combined chemical fertilizer (12.9 Mg ha(-1)). The effect of nutrient sources on cellulose, hemicellulose and lignin content was not significant at both locations. Cumulative biomass from twice harvest was similar to single harvest except in 2011 due to dry weather after the first cut. The cellulose and lignin content were significantly higher for single harvest compared with twice harvest at both locations. Published by Elsevier Ltd.