Zero liquid discharge is increasingly adopted as an objective for waste treatment process. The objective of this study was to increase the feed total solids (TS) and the organic loading rate (OLR) fed to a novel psychrophilic (20 degrees C) dry anaerobic digestion (PDAD). Duplicate laboratory-scale bioreactors were fed cow feces and wheat straw (35% TS in feed) at OLR of 6.0 g TCOD kg(-1) inoculum d(-1) during long-term operation (147 days consisting of 7 successive cycles). An overall average specific methane yield (SMY) of 151.8 +/- 7.9 N L CH4 kg(-1) VS fed with an averaged volatile solids removal of 42.4 +/- 4.3% were obtained at a volatile solids-based inoculum-to-substrate ratio (ISR) of 2.13 +/- 0.2. The operation was stable as indicated by biogas and VFAs profiles and the results were reproducible in successive cycles; a maximum SMY of 163.3 +/- 5.7 N L CH4 kg(-1) VS fed was obtained. Hydrolysis was the reaction limiting step. High rate PDAD of 35% TS dairy manure is possible in sequential batch reactor within 21 days treatment cycle length. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.