A microbial fuel cell (MFC) with biological Fe(III) reduction was implemented for simultaneous ethanolamine (ETA) degradation and electrical energy generation. In the feasibility experiment using acetate as a substrate in a single-chamber MFC with goethite and ammonium at a ratio of 3.0 (mol/mol), up to 96.1% of the ammonium was removed through the novel process related to Fe(III). In addition, the highest voltage output (0.53 V) and maximum power density (0.49 W m(-2)) were obtained. However, the ammonium removal and electrical performance decreased as acetate was replaced with ETA. In the long-term experiment, the electrical performance markedly decreased where the voltage loss increased due to Fe deposition on the membranes. (C) 2015 Elsevier Ltd. All rights reserved.