Graphitic biochar (BC) was generated using high temperature gasification and alkaline post-treatment (BCw) of wood-based biomass. The BCw was evaluated as a manganese oxide electrocatalytic support (MnO/BCw) and microbial fuel cell (MFC) air cathode. Nano-structured MnO2 crystals were successfully immobilized on biomass-based graphitic sheets and characterized using physical, chemical, and electrochemical analyses. Cyclic voltammetry of MnO/BCw/Nafion inks showed electrochemical features typical of beta-MnO2 with a current density of 0.9 mA cm (2). BC showed satisfactory maximum power densities of 146.7mWm (2) (BCw) and 187.8Wm (2) (MnO/BCw), compared with Vulcan Carbon (VC) (156.8mWm (2)) and manganese oxide VC composites (MnO/VC) (606.1mWm (2)). These materials were also tested as oxygen reduction reaction (ORR) catalysts for single chamber MFCs inoculated with anaerobic sludge. Our results demonstrate that BC can serve as an effective, low cost, and scalable material for MFC application. Published by Elsevier Ltd.