To obtain a novel adaptable stain that can produce enantioselective l-lactic acid efficiently, reduce the operational cost of fermentation process and be suitable for scale production. Enterococcus faecium S.156 produced 126 g l-lactic acid/l with high optical purity (99.7 %), high productivity (5.25 g/l.h) and a conversion ratio > 90 %. l-lactic acid production remained steady from 32 to 40 A degrees C and at pH values from 5.5 to 6.5. O-2 made no difference to both biomass growth and the l-lactic acid fermentation. 8 mg folic acid/l combined with 2 mM Fe2+ substituted for 6 g yeast extract/l, which is a cost-saving. The special characteristics and economic traits of E. faecium S.156 make it suitable for industrial-scale production of l-lactic acid.