In this study, a dual-chamber MFC was applied to simultaneously remove sulphide in wastewater and generate clean energy by using a mixed culture of microorganisms as active biocatalysts. The influences of three different concentrations of sulphide (0.1, 0.8, and 1.5 g/L-1) on MFC performance and their removal were examined. Anode potential was controlled by the concentration of sulphide in the compartment. Moreover, the influence of algae as a biocathode on produced power and current of MFC was investigated. A significant increase in the maximum power and current density in MFC indicated the remarkable impact of using a biocathode. Biocatalyst activities and sulphide oxidation in anaerobic conditions were indicated via cyclic voltammetry curves to confirm the target of the research.