We report the first example of enzymatic synthesis of lutein dipalmitate in organic solvents by using a commercial immobilized Candida antarctica lipase B (Novozyme 435) as the catalyst. At the optimized condition, by using 20 mg/mL of lutein, 100 mg/mL of vinyl palmitate as the substrates, and 20 mg/mL of Novozyme 435 as the catalyst, the enzymatic synthesis in toluene achieved a yield of lutein dipalmitate of 83 % after 8 h reaction at 60 A degrees C. The stability of the enzyme was demonstrated by over 80 % conversions for ten batches. A commercial immobilized Candida antarctica lipase B (Novozyme 435) was applied to catalyze the transerterification reaction between lutein and vinyl palmitate, achieving a yield of lutein dipalmitate of 83 % after 8 h reaction at 60 A degrees C. The enzymatic synthesis of lutein dipalmitate received more than 80 % conversion for each batch during the 10 cycles of reuse.