Developing efficient methods for the production of biotechnologically generated products is the goal of various current research approaches. One issue related is the extraction of biomass from aqueous fermentation broths for the isolation of respective products. Based on the high cell wall stability of available microorganisms and the corresponding high energy for cell disruption, extremely stable emulsions result after the indispensable cell disruption in aqueous environment. Therefore, the cell material is often dried and the product is extracted by means of organic solvents from the biomass, which makes these processes difficult both from the economic and toxicological sides. Based on the example of an extraction of polyunsaturated fatty acids from microalgae, a systematic approach is presented for the characterization of emulsion stability and development of process concepts for the subsequent solvent-free product recovery.