A real biogas effluent was desulfurized using an anoxic biotrickling filter at pilot scale. Guidelines and recommendations have been proposed to achieve the correct inoculation and biofilm development. The hydrogen sulfide inlet concentration (4100-7900 ppm(v)) was not controlled. The operational variables studied were the hydrogen sulfide inlet load (37-149 gS m(-3) h(-1)), biogas flow rate (1-3.4 Nm(3) h(-1)) and pH (6.8-7.4). Moreover, three nitrate-feeding modes were studied: manual, continuous and automated. Without the addition of nutrients to the nitrate solution, a removal efficiency greater than 95% was obtained for loads in the range 33-55 gS m(-3) h(-1) along with an elemental sulfur percentage of 85 +/- 5%. The nitrate solution was mainly composed of NaNO3 (500 g L-1), the macronutrients KH2PO4 (10 g L-1), NH4Cl (5 g L-1) and MgSO4 center dot 7H(2)O (4 g L-1), and trace elements. The critical elimination capacity was 94.7 gS m(-3) h(-1) (RE >99%) on day 119 and the maximum elimination capacity was 127.3 gS m(-3) h(-1) (RE = 92.6%) on day 122. The recommended operational conditions for start-up are an inlet load of 100 gS m(-3) h(-1), a pH set-point of 6.8 to reduce sulfide accumulation and nitrate-feeding automated by oxide reduction potential. (C) 2015 Elsevier B.V. All rights reserved.