Platinum nanosheets (Pt NSs) were fabricated on fullerene (C-60) decorated glassy carbon electrode (GCE) (denoted as Pt NSs/C-60/GCE) through a simple potentiostatic deposition method. In the coexistence of ascorbic acid (AA), dopamine (DA) and uric acid (UA), the as-prepared Pt NSs/C-60/GCE electrode exhibited three well-resolved voltammetric peaks (Delta EAA-DA = 176 mV, Delta EDA-UA = 132 mV, Delta EAA-UA = 308 mV) in the differential pulse voltammetry (DPV) measurements, allowing a simultaneous detection of these biomolecules. There are linear relationships between current intensities and concentrations in the region of 10-1800 mu M (AA), 0.5-211.5 mu M (DA), and 9.5-1187 mu M (UA), and the limits of detection (LOD) (S/N = 3) are down to 0.43 mu M, 0.07 mu M and 0.63 mu M for AA, DA and UA, respectively. The as-prepared Pt NSs/C60/GCE electrode displayed a good reproducibility and storage stability and was successfully used for detection of AA, DA and UA in real plasma and urine samples. (C) 2015 Elsevier Ltd. All rights reserved.