The novel hybrid nanomaterial catalyst of multi-wall TiO2 nanotube supported Ni(OH)(2) nanoparticles (Ni (OH)(2)/TNTs) was prepared through hydrothermal method and investigated as anode electro-catalysts for glucose oxidation. The nanostructure was characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), thermogravimetry-differential thermal analysis (TGA) and nitrogen adsorption-desorption (BET-BJH). The electrochemical performance was measured by a range of electrochemical measurements. Compared with Ni(OH)(2), the current density of Ni(OH)(2)/TNTs modified GC electrode increased 5.9 times in 0.1M NaOH solution. The results indicated that the synthesized nanoparticles exhibited good electro-catalytic activity and stability for glucose oxidation. Meanwhile, the hybrid nanomaterial of Ni(OH)(2)/TNTsmay be a potential candidate catalyst for direct glucose fuel cell. (C) 2015 Elsevier Ltd. All rights reserved.