Printed sensor has been introduced as a rapid and cost-effective platform for biomedical applications, especially for wearable biosensing and point-of-care diagnosis. In this paper, we reported a tumor cell density monitoring device with printed interdigital electrodes on a plastic film. Numerical simulation of electrical impedance spectroscopy shows good agreement with the experimental measurement of DI water and PBS solution. Different concentrations of cancer cells were used to demonstrate the capability of cell culture monitoring. The developed device is highly cost-effective and easy for fabrication, which may have great potentials in low-cost analysis of biological cells. In a word, this paper shows a more rapid and simple cell counting method as compared to tedious microscope cell counting.