Gas hydrate inhibition is a prime focus of industrial hydrocarbon research as pipeline blockages can be costly and dangerous. Historically, many chemical species have been studied for their effects on the hydrate formation process. One of the most investigated compounds in the kinetic hydrate inhibitor (KHI) category is polyvinylpyrrolidone (PVP). While the effects of PVP on hydrates are well-documented, the mechanism that defines its function is still not completely understood. To obtain further insight into its behavior, bulk liquid samples in a PVP-containing system were extracted at six specific times throughout a methane hydrate formation process. The effect of PVP loading concentration was also investigated. It was found that as time progressed, concentration of PVP in the liquid decreased, suggesting that PVP binds to the surface of growing hydrates. Furthermore, this decrease in concentration was more prevalent in situations where lower initial PVP loadings were used.