Chemical characterization of Jatropha curcas seeds is supposed to be an interesting tool to extend the knowledge on alternative products of this plant, which is of great economic interest. Chemical composition of seed oil reflects the quality of produced biodiesel. The seed oils of 6 jatropha (among 30) biotypes were evaluated for their oil quality parameters: oil content (38-41%), acid value (1.22-13.40 mg/g), free fatty acid (0.06-3.50%), iodine value (115.48-163.37 mg/g), and viscosity (0.6320-0.7431). Significant differences among biotypes were observed in oil yield and biochemical parameters. The variability among the biotypes indicate a good scope of genetic gain through selection.