CO2 flooding is a proven enhanced oil recovery (EOR) technique and is also considered as a potential method for CO2 sequestration. Despite having successful field trials on CO2 EOR, the effects of reservoir wettability and permeability heterogeneity on the efficiency of miscible CO2 flooding are not well understood. In this work, laboratory investigations have been carried out to evaluate the influence of these properties on the miscible CO2 EOR performance. The wettability of hydrophilic Berea core samples was altered to be oil-wet by vacuum saturation of the clean and dry core samples with n-hexadecane. The permeability heterogeneity was obtained by combining two half pieces of axially split water-wet core samples of different permeabilities. Core flooding experiments were conducted for n-hexadecane - synthetic brine - CO2 systems at 1400 psig backpressure to achieve minimum miscibility pressure (MMP) of CO2 in n-hexadecane at the test temperature (24 +/- 1 degrees C). It was found that wettability strongly influences CO2 EOR. For the alternate cases of previously brine flooded (to remaining oil saturation) oilwet and water-wet core samples, five pore volumes (PVs) of CO2 recovered 100% and only 43% of remaining oil in place (ROIP) respectively. Three PVs of CO2 could recover only about 0-5% ROIP from the split core samples. The mechanisms underlying these results are discussed. This study sheds light on the significant influence of reservoir wettability and permeability heterogeneity on the performance of miscible CO2 EOR. (C) 2015 Elsevier Ltd. All rights reserved.