In the present work, two types of cost-effective bio-electrodes were fabricated by immobilizing Laccase on commercially available pencil graphite leads, and their electro catalytic activity have been examined. The first type of electrode (E-1) was fabricated by electro polymerization of polyaniline on pencil graphite leads on which multi walled carbon nanotubes (MWCNT) was immobilized by covalent bonding, while the second type of electrode (E-2) was fabricated by direct coating of MWCNT on pencil graphite leads. Two types of bio-cathodes were fabricated by immobilizing Laccase on the two types of electrodes by covalent bonding. The scanning electron microscopy (SEM) revealed three dimensional porous networks providing large surface area for enzyme immobilization. The maximum open circuit potential (OCP) for E-1 and E-2 bio-cathodes were measured 0.58 V and 0.60 V respectively vs Ag/AgCl reference electrode. The maximum current density observed for E-1 and E-2 electrodes were 295.7 mu Acm(-2) and 228.94 mu Acm(-2) respectively. Both electrodes exhibited excellent stability, conserving more than 75% of its optimum activity during the period of measurement. Copyright (C) 2015, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.