AimsRed propolis is a resinous product popularly consumed in Brazil as it improves health, and it is considered a nutraceutical. The objective of this study was to test the antimicrobial activity of eight samples of red propolis from Brazil and Cuba to assess the possibility of application of this natural product as an antimicrobial agent, along with a study of its cytotoxic activity against non-tumor cell lines to evaluate at which concentrations it could be safely used. Methods and ResultsThe chemical profile of the samples was evaluated by UHPLC-MS. All the samples presented antimicrobial activity which was tested using agar diffusion and serial dilution methods; and these samples displayed a better activity against most Gram-negative bacteria with minimum inhibitory concentration (MIC) in the range between 625gml(-1) and 500gml(-1). However our studies also revealed an inherent cytotoxic effect against HaCaT human keratinocytes and BALBc 3T3. ConclusionsTo have a noncytotoxic and safe use of red propolis, it is necessary to use a concentration below the IC50 cytotoxic values. Significance and Impact of the StudyThe traditional use of propolis does not necessarily guarantee its safety. The evaluation of the safety of bioactive natural products should always be considered together with the evaluation of the activity.