AimsHuman norovirus (HuNoV) is estimated to cause 19-21 million illnesses each year in the US. A major limitation in HuNoV research is the lack of an invitro culture system; therefore, surrogate viruses including murine norovirus (MNV) and feline calicivirus (FCV) are used to study HuNoV. Here, we aim to establish the physiochemical properties of Tulane virus (TV)a newer HuNoV surrogate. Methods and ResultsFor thermal inactivation, TV was exposed to 37 degrees C for 2h, and 56, 63 and 72 degrees C for 30min. For ethanol tolerance, TV was treated with 60, 70 and 90% ethanol at room temperature (RT) for 5min. Tulane virus pH stability at pH 2, 3, 7, 9 and 10 was performed at RT for 90min. At 37 degrees C, there was no significant reduction in TV after 2h. However, at 56, 63 and 72 degrees C, D-values of 403, 118, and 024 min, were calculated respectively. The D-values obtained for TV ethanol tolerance were 146, 193, and 035 min at 60, 70 and 90% respectively. Less than 1 log(10) plaque forming units (PFU) reduction was observed for TV at all pH levels except pH 10 where about a 2-log(10) PFU reduction was observed. Tulane virus was also tolerant to chlorine disinfection on a solid surface with D-values of 1582 and 542min at 200 and 1000ppm respectively. ConclusionsTulane virus is likely a suitable surrogate to study HuNoV thermal stability as well as ethanol tolerance below 90%. Tulane virus also is a promising surrogate to study HuNoV pH stability and chlorine tolerance. Significance and Impact of the StudyBased on current work, invitro studies demonstrate that TV is an overall more conservative and suitable surrogate for the study of HuNoV physicochemical properties.