Journal of Bioscience and Bioengineering, Vol.119, No.4, 427-429, 2015
Enhanced cellular content and lactate fraction of the poly(lactate-co-3-hydroxybutyrate) polyester produced in recombinant Escherichia coli by the deletion of sigma factor RpoN
A new approach at the transcriptional level was applied to lactate-based polyester production. Four sigma factor disruptants, Delta rpoN, Delta rpoS, Delta fliA and Delta fecl, of Escherichia con were used as hosts for poly(lactate-co-3-hydroxybutyrate) production from glucose. Among them, Delta rpoN caused dual positive effects of polymer production, enhanced cellular content and lactate fraction. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.