Characterizing activities of eukaryotic-like protein kinases with atypical catalytic loop motifs from Myxococcus xanthus

Kimura Y; Urata M; Okamoto R

Journal of Bioscience and Bioengineering, Vol.119, No.5, 511-514, 2015

DOI10.1016/j.jbiosc.2014.10.002 Export Citation

Characterizing activities of eukaryotic-like protein kinases with atypical catalytic loop motifs from Myxococcus xanthus

Kimura Y, Urata M, Okamoto R

Myxococcus xanthus has eukaryotic-like protein kinases (EPKS) with different atypical catalytic loop motifs. Seven out of 14 recombinant M. xanthus EPKs containing atypical motifs in the catalytic loop showed protein kinase activity against myelin basic protein and four autophosphorylated EPKS were detected using anti-phosphotyrosine antibody by western blotting. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Myxococcus xanthus;Eukaryotic-like protein kinase;Dual-specificity kinase;MBP kinase activity;Atypical catalytic loop