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Journal of Bioscience and Bioengineering, Vol.120, No.1, 117-119, 2015
Enzyme assay for pyridoxal 5'-phosphate by apo-D-amino acid aminotransferase
D-Amino acid aminotransferase (D-AAT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme. n-AAT from thermophilic. Bacillus sp. YM-1 was inactivated by ultraviolet irradiation. The activity was restored by the addition of PLP and showed a linear correlation with respect to PLP concentrations in the range of 10-40 nM. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
Keywords:D-Amino acid aminotransferase;Pyridoxal 5 '-phosphate;Ultraviolet irradiation;Apoenzyme;Enzyme assay