Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway

Sato Y; Sugimoto N; Higashiyama T; Arata H

Journal of Bioscience and Bioengineering, Vol.120, No.6, 697-700, 2015

DOI10.1016/j.jbiosc.2015.03.021 Export Citation

Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway

Sato Y, Sugimoto N, Higashiyama T, Arata H

We developed two types of artificial platforms, T-junction and crossroad microchannel devices, and obtained guidance response ratio of pollen tubes to the female tissue as 56-57%. The crossroad device was also able to collect the attracted pollen tubes with high purity, which is useful for future omics analysis. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Plant reproduction;Pollen tube;Ovule;Pollen tube guidance;Torenia fournieri;Purification;Polydimethylsiloxane;Microchip;Microchannel;Micro-electro-mechanical system