A novel porous filter material was fabricated by element reactive synthesis method. The synthesized porous Ti3SiC2 membrane material presents a microstructure of both large substrate pores and small membrane pores, indicating large filtration flux and high filtration accuracy simultaneously. Microorganism filtration properties of the porous Ti3SiC2 membrane material were investigated by filtering the Escherichia coli suspension, which demonstrates excellent performance of the porous materials on filtration.