A novel braid reinforced (BR) cellulose acetate (CA) hollow fiber membrane consisting of separation layer and 'hybrid' braid was reported in this study. The 'hybrid' braid containing CA and polyacrylonitrile (PAN) fiber not only provided the membrane well interfacial bonding state but also overcame the negative effect of CA fiber's swelling on membrane permeability. The influences of braid composition and CA concentration on the structure and performance of BR CA membranes were investigated. There were two kinds of interfaces between the braid and the separation layer, which were named homogeneous-reinforced (HMR) interface and heterogeneous-reinforced (HTR) interface. Taking into account both interfacial bonding state and membrane permeability, the best ratio of the fibers in the braid was 2/1(CA/PAN). Increased CA concentration brought about reduced permeate flux and increased protein rejection. The BR CA membranes exhibited excellent anti-fouling properly with flux recovery rates higher than 80%. The tensile strength of BR CA hollow fiber membranes varied from 16.0 MPa to 62.9 MPa by adjusting the braid composition. The BR CA membrane showed similar performance with the commercial hydrophilic polyvinylidene fluoride (PVDF) hollow fiber membrane during the filtration of milk solution, and the flux could be recovered by chemical cleaning. (C) 2015 Elsevier B.V. All rights reserved.