The proton transfer (PT), process in 1-(2-hydroxy-6-chloro-phenyl)-3,5-dioxo-1H-imidazo-[3,4-b]-isoindole (ADCL) has been studied in three different copolymer -surfactant supramolecular assemblies prepared in aqueous 1% P123 triblock Copolymer micellar solution with Vatying, cOncentrations of sutfactants (sOdium dodecyl sulfate (sps), cetyltrimethylammonium bromide (CTAB) and triton-X-100 (TX 100)). The aim of the present study is to monitor the modulation of the PT process by changing the degree of rince liar hydration inside the P123 Micelle with the addition of the three different surfactants (two ionic and one non ionic), that is, in P123-surfactant aggregates. Besides, a comparative study has been done with these result S with those in water, pure P123 micellar medium and three different surfactants medium. The Micropolarity meaSurement and time-resolved fluorescence anisotropic meaSurements have been performed to evaluate the binCling location of the probe (AD CL) in the three 'different copolymer surfactant suptamolecular assemblies. It is found that the mictopolatity at the binding site of the molecule in the various environments largely influences the PT rate of ADCL. The 1-Yr rate is found to be the slowest in the P123 medium and in P123 surfactant aggregates the rate becomes faster as, the inicropoladty around the binding locations of the molecule in these aggregates is higher in comparison to that in P123 micelle.