Pt/graphene-TiO2 hybrid catalysts have been synthesized by a facile one-pot solvothermal method. The Structural properties of obtained Pt/graphene-TiO2 catalysts are characterized by X-ray diffraction (XRD), Energy dispersive analysis of X-ray (EDAX) and transmission electron microscopy (TEM),Interesting, TEM presents Pt nanoparticles seem preferentially to locate between TiO2 and graphene, forming the unique triple junctions structure. The electrochemical experimental results indicate that Pt/graphene-TiO2 catalyst exhibits 1.46 times higher activity for methanol electrooxidation than that of Pt/graphene and its stability is improved by 15% as compared with Pt/graphene. Moreover, performance of Pt/graphene-TiO2 hybrid catalyst is evaluated by the Single Fuel Cell Tests for the first time. Single fuel cell tests show the maximum power density of the direct methanol fuel cell using Pt/graphene-TiO2 as the anode catalyst is increased by 55% compared with that using Pt/graphene catalyst at the same operating conditions. The significantly enhanced electrochemical performance can be ascribed to (1) the synergetic effect between Pt, graphene and TiO2; (2) strong metal-support interaction (SMSI) between Pt nanoparticles and TiO2. These findings suggest their great potential applications in fuel cells. (C) 2015 Elsevier B.V. All rights reserved.