A novel platinum decorated CuO nanoleaves were synthesized through a facile co-precipitation method and further utilized as a sensing platform for glucose based on immobilization of Pt-CuO hybrid nanostructures with Nafion matrix. The prepared composites were characterized by X-ray Diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The modified electrode exhibits excellent electrocatalytic activity towards glucose as probed by cyclic voltammetry (CV) and chronoamperometry (CA) techniques. Comparing with pure CuO/Nf/GCE, Pt-CuO/Nf/GCE, as non-enzymatic electrode materials, exhibited a good response towards glucose with linear range 2-1000 mu M with a higher sensitivity of 1681.7 mu A mM(-1) cm(-2) and detection limit of 11 mu M. The excellent electrocatalytic performances could be attributed to the Cu(II)/Cu(III) multiple oxidation states system could promote the redox reactions between electrode materials and glucose, and the electroactive sites became more active due to the synergic effect. The results, high sensitivity, excellent selectivity and long term stability showing that, Pt-CuO is a promising material for the future development of nonenzymatic glucose sensors. (C) 2015 The Electrochemical Society. All rights reserved.