Micropatterns which can be controlled precisely were obtained on titanium (Ti) surface by femtosecond lasers (FSL) ablation. The in-situ deposition of bioactive calcium phosphate (Ca/P) phase and collagen hybrid was achieved on Ti surface to form filamentous structure combined with nanoparticles, during the FSL treatment. The amount of deposited Ca/P phase increased with the laser energy during the whole process. While for the deposited collagen amount, it increased with the rise of the laser energy initially, and then decreased with the increase of laser energy. The FSL induced micropatterns and in-situ deposition of Ca/P phase and collagen are expected to result in excellent bioactivity and biocompatibility of the Ti implant. (C) 2015 Elsevier B.V. All rights reserved.