Mg2BO3F:Dy phosphor is a low Z material with an effective atomic number 9.92. This phosphor was synthesized by two different methods, because the solid state method failed to produce single phase Mg2BO3F:Dy phosphor. This study reports a comparative investigation of gamma-ray and C5+ ion beam impact on thermoluminescence (TL) properties of Mg2BO3F:Dy phosphor. TRIM/SRIM calculations were performed to correlate the changes in 11. properties of Mg2BO3F:Dy phosphor. Phosphor possesses good response towards gamma-rays as well as C5+ ion beam irradiation. TL glow curves of the C5+ ion irradiated phosphor show a small shift in the position of the glow peaks towards the lower temperature side. With an increase in energy of the ion beam increase in TL intensity was observed. This increase in the TL efficiency with a decrease in linear energy transfer (LET) agrees well with the results reported in literature. Trapping parameters of the gamma-ray and C5+ ion beam irradiated phosphor were calculated from the TL data using computerized glow curve deconvolution method. The trapping analysis suggests that ion beam irradiation causes only minor changes in the activation energy. The different types of defects responsible for TL glow curves were identified using the EPR technique. (C) 2015 Elsevier B.V. All rights reserved.