We investigated on the photoisomerization of azobenzene derivatives in liquid crystalline matrix that is 4-pentyl-4-bisphenyl carbonitrile (5CB) for holographic memory. In order to investigate of photoisomerization of azobenzene derivatives at the air/water interface, the azobenzene derivative having side chain monomer with alkyl chain was synthesized. The azobenzene derivatives in liquid crystalline matrix were characterized by the measurements of UV-vis spectroscopy, photorefractive index by two-beam coupling (2CB).