The palaeobiological record of 12 million to 7 million years ago (Ma) is crucial to the elucidation of African ape and human origins, but few fossil assemblages of this period have been reported from sub-Saharan Africa. Since the 1970s, the Chorora Formation, Ethiopia, has been widely considered to contain similar to 10.5 million year (Myr) old mammalian fossils(1-7). More recently, Chororapithecus abyssinicus, a probable primitive member of the gorilla clade(6), was discovered from the formation. Here we report new field observations and geochemical, magnetostratigraphic and radioisotopic results that securely place the Chorora Formation sediments to between similar to 9 and similar to 7 Ma. The C. abyssinicus fossils are similar to 8.0 Myr old, forming a revised age constraint of the human-gorilla split. Other Chorora fossils range in age from similar to 8.5 to 7 Ma and comprise the first sub-Saharan mammalian assemblage that spans this period. These fossils suggest indigenous African evolution of multiple mammalian lineages/groups between 10 and 7 Ma, including a possible ancestral-descendent relationship between the similar to 9.8 Myr old Nakalipithecus nakayamai(8) and C. abyssinicus. The new chronology and fossils suggest that faunal provinciality between eastern Africa and Eurasia had intensified by similar to 9 Ma, with decreased faunal interchange thereafter(9-12). The Chorora evidence supports the hypothesis of in situ African evolution of the Gorilla-Pan-human clade, and is concordant with the deeper divergence estimates of humans and great apes based on lower mutation rates of similar to 0.5 x 10(-9) per site per year (refs 13-15).