In the present study, we investigated the effect of pH on phase transition of TiO2 nanoparticles synthesized by sol-gel protocol and identification by various techniques as x-ray diffraction, energy dispersive x-ray spectroscopy (EDX), Raman spectroscopy, scanning electron microscopy, etc. Corresponding TiO2 phase change from rutile to anatase and mixture of both anatase and rutile phases by tuning the pH of the reaction mixture is clear from x-ray diffraction studies. The presence of (101) and (110) planes corresponded to anatase and rutile phases of TiO2, respectively.