Plant protein isolates (wheat gluten, soy, and pea protein) were treated in a thermoplastic extrusion process, yielding protein based bioplastic material. The application of glycerol as plasticizer in amounts of 50-67% of total mass and high temperatures above 140 degrees C led to smooth and homogeneous extrudates. Viscosity measurements of the protein melt under extrusion conditions confirmed the thermoplastic behavior, obeying the power law with power law indices of 0.31-0.4. Chemical changes in the material were qualitatively shown by amino acid analysis and fluorescence measurement. High moisture sensitivity and low mechanical stability of the extrudates, as determined by solubility, water vapor permeability, sorption isotherms, and tensile strength, can be partially ascribed to the high glycerol content. The application of pure protein bioplastics for technical purposes, e.g., as packing material, is discussed on basis of the presented data considering stability, appearance, and long time storage. (c) 2014 Society of Plastics Engineers