Many LCA based viability studies have already been done for the production of green diesel from microalgae, still a comprehensive LCA, has not yet been undertaken. Current study aims to find out if the Net Energy Balance(NEB) can further be increased by using a combination of many available agronomical practices & the techniques of production of green diesel from microalgae. The results show that neither open raceway pond nor Photobioreactor routes (Wet and dry routes) yield positive energy balance. The production of green diesel via open raceway pond, both in dry as well as wet route, have less negative NEB and comparatively higher NER than the photobioreactors. Comparison says that open raceway pond dry route has slightly higher value for NER than the wet route. Even with the best possible route (open raceway pond dry route), the total energy use is almost 5 times more than the energy produced, with a negative NEB of 4.07 MJ & very low NER value of 0.20. Study concludes that R & D in the area of green diesel production from microalgae has yet to go a long way & has a huge scope to further lower its input energy demand for biofuel production. (C) 2015 Elsevier Ltd. All rights reserved.