The objective of this paper is to conduct Life Cycle Assessment (LCA) and Environmental Life Cycle Costing (ELCC) studies for lignocellulosic bioethanol blends [E10 and E85 (10% and 85% by volume of bioethanol with gasoline, respectively)] and conventional gasoline (CG). To compare the environmental performance and economic advantage of the selected fuel blends, the impact potentials and the cost of fuel applications per kilometer by a middle size car was evaluated. According the LCA results, one kilometer driven by E10 and E85 fueled vehicles could reduce the greenhouse gas (GHG) emissions by 43% and 47% and ozone layer depletion emissions by 3% and 66%, respectively, relative to CG. However, shifting from gasoline to bioethanol increases the emissions that contribute to eutrophication and photochemical ozone depletion. In terms of acidification potential, E85 shows a more favorable result relative to E10 and CG. According to the ELCC analysis, E85 fuel use provides a 23% lower driving cost relative to CG based on a-1 km driving distance. The results showed that E85 seems to be the best alternative in terms of both GHG emission and fuel production cost reduction compare to CG. (C) 2015 Elsevier Ltd. All rights reserved.