We report the investigation findings of monolayers behaviour of nile red new synthesized amphiphilic analog at the air/water interface and its spectral-luminescent properties in solvents of various polarity and in Langmuir-Blodgett films consisting of dyes and stearic acid mixture. It is shown, that electronic absorption spectra of films depends on the presence of two monomer types of various spatial conformations. The position of the films fluorescence band is identical to a case of polar medium.