Ultrathin film of multi-walled carbon nanotubes (MWCNTs) was fabricated using a liquid-liquid interface. Water dispersible MWCNTs, which were synthesized using an anodic aluminum oxide film as a template were used as a water phase. Hexane solution was added to the water dispersion as an oil phase. The MWCNTs were assembled at the liquid-liquid interface by adding 10 vol% of alcohol (methanol, ethanol, and 2-propanol) to the MWCNTs water dispersion/hexane solution. The assembled film was transferred to a solid substrate and the effect of alcohol type to the film properties was discussed.