Sensitivity of a glucose biosensor based on conducting polymer micro-tubule was enhanced by introducing a Pt transducer layer. Electrochemical template synthesis using polycarbonate membrane was used to fabricate the micro tubule. Glucose oxidase from Aspergillus Niger was caged into the micro tubule structure by capping the mouth of the tubule with a nano porous polycarbonate membrane. This capped tubule structure worked as a cell-array retaining enzyme inside and as a sensing current collector for the electrochemical reaction of the species retained inside simultaneously. The nano pores of the capping membrane were filled with poly (1,3-phenylenediamine) by using electrochemical polymerization. Introducing a Pt transducer layer at the bottom side of the template enhanced the sensitivity of the sensor about sixty times compared to the one having Au layer. The larger the pore size of the template was, the faster was the response and the higher was the detection current because of the faster material transport into and out of the cells.