An ITO electrode based neuronal cell chip was developed to electrochemically monitor the effect of environmental toxins on PC12 cell neurite outgrowth. PC12 cells were immobilized on an ITO electrode coated with a synthetic oligopeptide, which increased the cellular attachment and stability on the electrode surface. The undifferentiated PC12 cells on the ITO electrode were treated with the nerve growth factor to induce neurite outgrowth. The differentiation level of PC12 cells were determined by confocal microscopy. The effects of Bisphenol A on the cells were examined by cyclic voltammetry. We found that cyclic voltammetric peak current decreased with the concentration of Bisphenol A because of the decreased cell viability. This study has a number of potential applications in cell based biosensors and neuronal prosthetic devices.