The objective of the present work was to prove that the spectral features of chlorophyll a allow its use as a sensor for physical properties of liposomes. For this purpose, UV-VIS absorption, fluorescence emission and dynamic light scattering measurements were recorded and analyzed comparatively. Multilamellar and unilamellar vesicles with Chla have been prepared by a mechanical dispersion method and a further sonication treatment for obtaining unilamellar vesicles. The Chla - liposomes were prepared using an appropriate molar ratio and three types of lipids. It was confirmed that Chla - based model system can be used to assess specific molecular interactions in the liposome bilayer.