Glucose oxidase (GOX)/poly(3,4-ethylenedioxythiophene) (PEDOT) electrode was prepared for glucose sensing. PEDOT thin film was first electrochemically or chemically polymerized on Pt substrate, followed by dipping it into GOX solution to immobilize GOX on PEDOT surface. It was observed that GOX was adsorbed much more efficiently on the chemically polymerized PEDOT than on the electrochemically polymerized PEDOT. The cyclic voltammetry of the thin film electrode showed a clear anodic peak at -0.43 V. It was also observed that the anodic current monotonically decreased with the increase of glucose concentration dissolved in the electrolyte solution, implying that the electrode can be applied as a simple glucose sensor.