A fluorenyl group-containing polyacetylene, poly(9-ethynyl-9-fluorenol) was prepared by the polymerization of 9-ethynyl-9-fluorenol (EF) by [(nbd)RhCl](2). The polymerization proceeded well in homogeneous manner to give in high yield of polymer. The chemical structure of poly(EF) was characterized by such various instrumental methods as NMR, IR, and UV-visible spectroscopies to have the poly(EF) have a conjugated polymer backbone system with the designed fluorene groups. Poly(EF) showed edge range of UV-visible absorption spectrum about 600nm and the band gap of poly(EF) was 2.20eV. The maximum photoluminescence peak was observed at 431nm.