The chemical composition of the fatty acid methyl esters (FAMEs) of flowering tops of Hypericum scabrum, H. scabroides and H. amblysepalum were identified by gas chromatography. In this work 5 FAMEs for H. scabrum, 9 FAMEs for H. scabroides and 8 FAMEs for H. amblysepalum were identified. The major components were alpha-linolenic (48.60%), linoleic (32.53%) and oleic (11.45%) acids in H. scabrum. Alpha-linolenic (29.84%), palmitic (27.90%) and oleic (16.49%) acids were present in H. scabroides and alpha linolenic (41.79%) and palmitic acids (32.28%) were found in H. amblysepalum.