From the methanolic extract of the underground parts of Globularia cordifolia, a new neolignan digalycoside, dehydrodiconiferyl alcohol 9-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranoside (1) was isolated along with a known neolignan glycoside, dehydrodiconiferyl alcohol 9-O-beta-D-glucopyranoside (2). In addition, 2 flavone glycosides, chrysoeriol 7-O-beta-D-allopyranosyl-(1-->2)-beta-D-glueopyranoside (3), Stackyspinoside (4) and 5 phenylethanoid glycosides, verbascoside (5), isoverbascoside (6), leucosceptoside A (7), martynoside (8) and rossicaside A (9) were obtained and characterized. The structures of the isolates were established by 1D and 2D NMR spectroscopy in combination with IR, UV and MS analysis.