From the aerial parts of Asperula arvensis L. 9 known flavonol glycosides, namely quercetin (1), isoquercitrin [= quercetin 3-O-beta-glucopyranoside] (2), hyperin [= quercetin 3-O-beta-galactopyranoside] (3), quercetin 7-O-beta-galactopyranoside (4), quercetin 4'-O-beta-galactopyranoside (5), isorhamnetin 3-O-beta-galactopyranoside (6), isorhamnetin 5-0-beta-galactopyranoside (7), dihydrokaempferol 7-4'-dimethylether 3-O-beta-glucopyranoside (8) and isorhamnetin 3-O-alpha-rhamnopyranosyl (1''' -> 6'')-beta-glucopyranosid (9), were isolated. The structures of the compounds were elucidated by high field 1D and 2D NMR and ESI-MS spectroscopies.