Synthesis of some novel 1-(3,4,5-trimethoxy) benzoyl-3-arylthiourea derivatives (1a-o) was accomplished in 2 steps. The synthetic route involves the reaction of 1-(3,4,5-trimethoxy) benzoyl chloride with potassium thiocyanate in 1: 1 molar ratio in acetone to afford the corresponding isothiocyante followed by treatment with suitably substituted anilines. The structures of the products were established by elemental analyses, IR, (1)H-and (13)C-NMR, and mass spectroscopy and for 1b and 1m from single crystal X-ray diffraction data. All of the synthesized compounds (1a-o) were screened for antibacterial activity against gram positive and gram negative bacterial strains and were found to exhibit low activity towards the tested microorganisms, compared to chloramphenicol, the standard drug.